Application

AOAC Official Method 989.13

Components

Test Units Antibody Iodine-Iodide solution Test trays

Features and Benefits

No equipment required to read the results

General description

The 1-2 Test is a rapid qualitative method for the detection of motile Salmonella in foods, food ingredients and environmental samples. It is based on the observation of Salmonella immobilized in motility medium by polyvalent H (flagellar) antibodies. Immobilization of motile Salmonella results in development of well-defined band of cells ImmunoBand. As an AOAC Official Method of Analysis, the 1-2 Test is one of the simplest rapid methods available for the detection of Salmonella.

Legal Information

1-2 Test is a registered trademark of BioControl Systems, Inc.

AOAC is a registered trademark of AOAC International

Principle

The assay incorporates immunodiffusion technology to provide accurate and rapid results following a simple 1 or 2 step enrichment protocol. If present,Salmonella move through the motility media as Salmonella -specific antibodies diffuse into the media from the opposite direction. The antibodies bind to the target Salmonella antigen to form a visually apparent immunoband indicating a positive test result.

Testing Method

Immunodiffusion